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Molecular weight determination can be achieved through techniques such as Size Exclusion Chromatography (SEC), Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry (MALDI-TOF MS), and Ultracentrifugation

You need the full picture: serum B12, methylmalonic acid, homocysteine, and if you can get it, holotranscobalamin

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Meanwhile, the physicochemical properties of peptides such as distribution, isoionic point and pH are required to be optimized

Autoimmune Conditions : Certain peptides can trigger immune responses, so those with autoimmune disorders should only proceed under close medical supervision